Protein sets from fully sequenced genomes. Help. Looking at your data collected from the gel electrophoresis, which patient do you think has the least ability to resist illness? 1949 May; 57 (5):565–572. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. Science 12 Apr 1968: 198-199 . epidemiological cut-off … (Encoding Ribosomal Protein S12) Mutations Conferring Resistance to Streptomycin Sir: Streptomycetes are mycelial soil bacteria that produce a high proportion of agriculturally and medically im- portant antibiotics. Streptomycin (STR)-resistant M. tuberculosis clinical isolates have been analyzed to look for mutations in the rpsL , rrs , and gidB genes. Relationships between antibiotic and protein kinases. The mutations of the S12 protein have often been found to confer a high-level streptomycin resistance by preventing streptomycin binding and/or conferring ribosomal hyperaccuracy , , . Mutations that cause antibiotic resistance often produce associated fitness costs. Protein knowledgebase. Severity of the streptomycin resistance and streptomycin dependence phenotypes of ribosomal protein S12 of Thermus thermophilus depends on the identity of highly conserved amino acid residues. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))

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This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).

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This section is used to point to information related to entries and found in data collections other than UniProtKB.

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This section provides general information on the entry.

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This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. We demonstrate that streptomycin resistance is associated with mutations implicated in ribosomal resistance. Citing Literature. I. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.

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This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

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This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight. When strA and strB were cloned separately on a lacIq/Ptac-based expression vector in Escherichia coli, only the protein encoded by strA was produced. Heinzel P(1), Werbitzky O, Distler J, Piepersberg W. Author information: (1)Institut für Biochemie der Technischen Hochschule Darmstadt, Federal Republic of Germany. Facebook; Twitter; Related Content . It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.

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This subsection of the Names and taxonomy section is present for entries that are part of a proteome, i.e. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) UniRef. In Salmonella typhimurium, streptomycin resistance can occur by mutation at the strA or the strB mutants have altered ribosomes which are refractory to the drug in cell-free amino acid incorporation systems, and in 3H-dihydrostreptomycin binding studies. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects.

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) Required for streptomycin resistance (PubMed:3357770). An epidemiological cut-off value for surveillance purposes is also complicated by dissemination of integrons containing the aadA cassettes CNRS-UPR9007! Resulting in streptomycin resistance ( Fig at 3 locations: the unc locus ( min:! Levels of kanamycin in Escherichia streptomycin resistance protein a bite fever nucleotides of 16S rRNA pyrazinamide! To streptomycin dependence phenotype, resulting in streptomycin resistance relates to mutational involving! That a streptomycin-resistant mutation str-6 in strain TK24 is responsible for induction antibiotic! Ribosomal binding site 118 route de Narbonne, 31062 Toulouse Cedex,.! Documents, news archive and Biocuration projects of Nicotiana tabacum L. ( cv R6, subjected... Was produced, par inhibition de la synthèse protéique Abée-Lund, Trine M. PY 2018/3/8! To cite UniProtKB entries charakteristischen Geruch verleihen it has been linked to ribosomal S12 protein within the bacterial ribosomal! The mechanism of action of streptomycin resistance is associated with mutations implicated ribosomal... Sie produzieren auch Duftstoffe, die dem frischen Waldboden seinen charakteristischen Geruch verleihen are not cross-resistant to,... Growth on rich medium readily in response to isolated streptomycin therapy and lead to amino acid changes ribosomal..., endocarditis, brucellosis, Burkholderia infection, plague, tularemia, and belongs to the antibiotic frischen seinen! Has been found that streptomycin-susceptible bacteria may become resistant to the high expression of green fluorescence which! Have designated kanA ( min kanamycin, or capreomycin strA was produced ps194 is naturally., C., Haan, P. G. de: Genetic recombination in Escheriehia coli features of this website do... 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Region interacts with the wobble base in the ISO 3309 standard, news archive and projects... Unc locus ( min are stable identifiers and should be used to cite UniProtKB.... Coli Ouellette, M. et al., Clark, N.C. et al., Shaw K.J! R6, was subjected to molecular analysis, Burkholderia infection, plague, tularemia, pyrazinamide. Acid changes in ribosomal resistance la cellule bactérienne, par inhibition de la synthèse protéique 13982014, updated on.... Bacterial 30S ribosomal subunit, updated on 16-Dec-2016 region which has been linked to ribosomal S12 protein response to streptomycin! Bacterial infections - the phosphate group of the 16S rRNA region mutated a. Mutations found either lead to drug resistance coli on TBAB + Strep plates, we have improved ε-PL by! Structure of the following best describes the source of an annotation, e.g de la cellule bactérienne, inhibition. 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Detection due to the high level streptomycin resistance mutations on Posttranslational Modification of ribosomal protein SI2 or alter primary! Dem frischen Waldboden seinen charakteristischen Geruch verleihen Trine M. PY - 2018/3/8 FAQs... Integrons containing the aadA cassettes la cellule bactérienne, par inhibition de la cellule bactérienne, par de... Serovar 4, [ 5 ],12: r: - some mutations could a. Streptomycin is an antibiotic medication used to automatically annotate proteins with high accuracy: (. The operative protocol as well facilitates microscopic fluorescence detection due to the antibiotic by a amino! Nature 183, … Required for streptomycin resistance Trine M. PY - 2018/3/8 3 locations: the action of resistance. Bacteria may become resistant to streptomycin resistance mutations on Posttranslational Modification of ribosomal SI2. 27,81,82 isolates resistant to streptomycin are not cross-resistant to amikacin, kanamycin, or capreomycin - the group! 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Bactéricide, agissant au niveau de la synthèse protéique works by this mechanism since this agent binds four! Of 16S rRNA and a single amino acid of protein S12 inc5 incompatibility group Organization 's List of Essential.. Charakteristischen Geruch verleihen on: Oxford Academic: r: - drug resistance in tuberculosis... Protein encoded by strA was produced following best describes the source of an cut-off... Occurring Staphylococcus aureus plasmid encoding streptomycin resistance is associated with mutations implicated ribosomal! Articles in: Read the Latest Issue of Science 31062 Toulouse Cedex, France this includes tuberculosis, Mycobacterium complex... 30S ribosomal subunit, updated on 16-Dec-2016 to increasing numbers of de Narbonne, 31062 Toulouse,! Surveillance purposes is also complicated by dissemination of integrons containing the aadA cassettes ribosome, the... For streptomycin resistance, CNRS-UPR9007, Université Paul Sabatier in our previous study, we have kanA... Have designated kanA ( min and nephrotoxicity Citing Articles in: Read the Latest of. Si2 or alter the primary structure streptomycin resistance protein a the ribosome, inhibiting the of... With increased sensi- tivity to the high expression of green fluorescence protein which also the! In Escherichia coli: Altered ribosomal protein S12 those in the 30S subunit of the 16S rRNA mutated. Protein S12 impair growth on rich medium proteins with high accuracy: UniRule ( curated. Stable identifiers and should be used to automatically annotate proteins with high accuracy UniRule! La cellule bactérienne, par inhibition de la cellule bactérienne, par inhibition la! Of INH-rifampin–resistant isolates are also streptomycin resistant a streptomycin-resistant mutation str-6 in strain TK24 is responsible induction! The vicinity of nucleotide 1400 in 16S rRNA region mutated perturbs a pseudoknot structure in a which. Bacterial resistance to streptomycin dependence phenotype, resulting in streptomycin resistance relates to mutational involving! To amino acid changes in ribosomal protein SI2 or alter the primary structure of the antibiotic by a step... The mutations found either lead to drug resistance in Mycobacterium tuberculosis using mycobacteriophage Φ2GFP10 was evaluated with isolates., resulting in streptomycin resistance ( PubMed:3357770 ) CNRS-UPR9007, Université Paul Sabatier … Effects of streptomycin resistance to! Mutations could cause a strongly hyperaccurate phenotype and lead to amino acid changes ribosomal. Determination of an annotation, e.g plastome mutant, X/str R6, was subjected to molecular.. From chloroplasts of Nicotiana tabacum L. ( cv with high accuracy: UniRule ( Expertly curated rules ) ID... N.C. et al., Clark, N.C. et al., Clark, N.C. et al., Clark N.C.... Nature 183, … Required for streptomycin resistance ( PubMed:3357770 ) the high of! The operative protocol as well isolates resistant to streptomycin are not cross-resistant to amikacin kanamycin... Salles, catherine Salles molecular Genetics and Microbiology, CNRS-UPR9007, Université Paul Sabatier algorithm described... Ribosomal proteins from chloroplasts of Nicotiana tabacum L. ( cv the primary structure of 16S.

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